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평가분야

  • Obesity Levels
  • Purpose
    - To analyze the body composition in vivo
    Evaluation Items
    - Whole body fat
    - Lean body mass
    Method
    - Non-invasively quantitate the signals for fat mass in mice or rats using 1H magnetic resonance spectroscopy (LF90-II)
    Characteristics
    - Fast and accurate in vivo measurement of fat content in mice or rats. Non-invasive anesthesia-free measurement. Minimum stress to the animals. Enables easy repetition for longitudinal study
  • Energy Balance
  • Purpose
    - To measure food intake and energy expenditure to analyze the cause of obesity
    Evaluation Items
    - Food consumption
    - Water consumption
    - Energy expenditure
    - Locomotor activity
    - Core temperature
    Method
    - Comprehensive Lab Animal Monitoring System(CLAMS)
    Characteristics
    - Simultaneous and continuous quantitative analyses of experimental animals. Enales the simultaneous analyses up to 16 mice and 8 rats.
  • Motor Ability
  • Purpose
    - measure the exercise ability of the animals on the treadmill and energy expenditure during exercise.
    Evaluation Items
    - Exercise capacity
    - Energy expenditure and RQ during exercise
    Method
    - Treadmill system
    Characteristics
    - Simultaneous measurements of four mice and four rats.
  • Impaired Glucose Metabolism
  • Purpose
    - Measurement of glucose tolerance or insulin sensitivity in vivo.
    Evaluation Items
    - Glucose profile
    Method
    - Glucose Tolerance Test (GTT)
    - Insulin Tolerance Test (ITT)
  • Measurement of Insulin Resistance and Metabolic Flux
  • Purpose
    - Measurement of insulin resistance and metabolic flux, which are the causes of type 2 diabetes and metabolic syndrome.
    Evaluation Items
    - Whole body insulin sensitivity (GINF)
    - Whole body and tissue specific glucose uptake, glycolysis and glycogen synthesis
    - Hepatic glucose production
    Method
    - Hyperinsulinemic-euglycemic clamp with radioisotope dilution method
    Characteristics
    - Quantitative measurement of whole body and tissue-specific glucose fluxes in mice or rats.
    - The most accurate method to measure insulin resistance in the liver and peripheral tissues.
  • Hypoglycemia
  • Purpose
    - Measurement of metabolic changes caused by hypoglycemia in the central nerve system and peripheral tissues.
    Evaluation Items
    - The effect of hypoglycemia on central and peripheral response and metabolism
    Method
    - Hypoglycemic clamp
  • Fatty Acid Oxidation Capacity
  • Purpose
    - Measurement of fatty acid oxidation capacity in the whole body, muscle and liver cells.
    Evaluation Items
    - In vivo whole body fat oxidation
    - Ex vivo muscle fat oxidation
    - Primary hepatocyte fat oxidation
    Method
    - infused or incubated with a 14C-Oleic acid to determine 14CO2 generation
    Characteristics
    - Accurate analysis of the whole body fatty acid oxidation capacity
  • MRS/MRI
  • Purpose
    - To analyze the tissue-specific concentration and temporal changes of the metabolites in vivo.
    Evaluation Items
    - 1H-MRS – Quantify fats in the liver and muscle
    - 13C-MRS – Measure glycogen creation and glycolysis in the muscle and liver through TCA cycle flux measurement
    - Evaluate the mitochondria function
    - 31P-MRS – Measure ATP generation rate in the muscle and liver
    - MRI – Quantitative measurement of liver/muscle/heart/whole-body fats
    - Quantitative measurement of drug distribution in tissues
    Method
    - Magnetic resonance spectroscopy (MRS) and magnetic resonance imaging (MRI)
    Characteristics
    - High-performance 9.4T animal MRS equipped with four receiver channels.